EFFECT OF EUGENOL EMULSION IN POLYSORBATE-80 ON CLINICAL STRAINS OF CANDIDA ALBICANS

Valentyna P. Polanska; Vira I. Fedorchenko; Svitlana V. Zachepilo; Nellia A. Bobrova

doi:10.21272/eumj.2021;9(3):238-246

Valentyna P. Polanska Microbiology, Virology and Immunology Department of the Poltava State Medical University, Poltava, Ukraine https://orcid.org/0000-0002-8727-9029
Vira I. Fedorchenko Microbiology, Virology and Immunology Department of the Poltava State Medical University, Poltava, Ukraine https://orcid.org/0000-0002-7981-3025
Svitlana V. Zachepilo Otorhinolaryngology and Ophthalmology Department of the Poltava State Medical University, Poltava, Ukraine https://orcid.org/0000-0002-2194-0611
Nellia A. Bobrova Microbiology, Virology and Immunology Department of the Poltava State Medical University, Poltava, Ukraine https://orcid.org/0000-0002-1071-5697

DOI: https://doi.org/10.21272/eumj.2021;9(3):238-246

Keywords: еugenol, polysorbate-80, otomycosis, C. albicans, colony-forming units, minimal inhibitory concentration, minimal fungicidal concentration

Abstract

Candida albicans occupies a dominant position in the list of causative agents of candidal lesions of the ear. The development of new antifungal agents, an alternative source of which can be herbal essential oils and their components, remains a priority. One such agent with antiseptic, anti-inflammatory, and analgesic action is eugenol which is a phenol substance. Therefore, this article was aimed to study the effectiveness of the antifungal action of eugenol emulsified in Polysorbate-80 against clinical strains of C. albicans isolated from patients with external otomycosis.

The study was performed using 6 clinical strains of C. albicans isolated from the ear canal of patients with otitis externa. The diagnosis of fungal disease was established based on the results of clinical and laboratory (microscopical and mycological) studies of pathological material.

Analysis of mycological research showed that mainly representatives of the genus Aspergillus and Penicillium were revealed and only in 7% there were Candida genus fungi. C. albicans remained the dominant species of clinical significance. The results of our studies showed a high level of antifungal activity of eugenol on all clinical strains of C. albicans, including a remarkable inhibitory and fungicidal effect. At postmycostatic concentrations, the eugenol caused partial inhibition of reproduction of the clinical strains of fungi, which was replaced by a subsequent increased cell reproduction rate.

So, the investigation has shown that C. albicans is the dominant species among fungi of the Candida genus in the structure of the microbial profile of otomycoses. The eugenol, emulsified in Polysorbate-80, has a high antifungal effect against clinical strains of C. albicans. At postmycostatic concentrations, the eugenol caused partial inhibition of reproduction of the clinical strains of fungi, which was replaced by a subsequent increase cell reproduction rate.

Author Biographies

Valentyna P. Polanska, Microbiology, Virology and Immunology Department of the Poltava State Medical University, Poltava, Ukraine

Candidate of Biological Sciences, Associate Professor of the Microbiology, Virology and Immunology Department of the Poltava State Medical University, Poltava, Ukraine

Тel.: 095 690 84 90, e-mail: imama1956@gmail.com

ORCID https://orcid.org/0000-0002-8727-9029

Scopus-Author ID 57204069048

Vira I. Fedorchenko, Microbiology, Virology and Immunology Department of the Poltava State Medical University, Poltava, Ukraine

Candidate of Biological Sciences, Associate Professor of the Microbiology, Virology and Immunology Department of the Poltava State Medical University, Poltava, Ukraine

Тel.: 066 507 07 59, e-mail: fedorchenko.vira@gmail.com

ORCID https://orcid.org/0000-0002-7981-3025

Scopus-Author ID 57018868700

Svitlana V. Zachepilo, Otorhinolaryngology and Ophthalmology Department of the Poltava State Medical University, Poltava, Ukraine

Candidate of Medical Sciences, Associate Professor of the Otorhinolaryngology and Ophthalmology Department of the Poltava State Medical University, Poltava, Ukraine

Тel.: 099 718 21 55, e-mail: s.v.Zachepylo@gmail.com

ORCID https://orcid.org/0000-0002-2194-0611

Scopus-Author ID 57200449771

Nellia A. Bobrova, Microbiology, Virology and Immunology Department of the Poltava State Medical University, Poltava, Ukraine

Candidate of Biological Sciences, Lecturer of the Microbiology, Virology and Immunology Department of the Poltava State Medical University, Poltava, Ukraine

Тel.: 066 740 9562, e-mail: nelbobrova52@gmail.com

ORCID https://orcid.org/0000-0002-1071-5697

Scopus-Author ID 7004546261

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