ASSOCIATION BETWEEN THE RS801460-POLYMORPHISM IN THE SRA1 GENE AND THYROID NODULES AMONG UKRAINIAN WOMEN WITH PROLIFERATIVE TYPE OF BENIGN BREAST DYSPLASIA WITHOUT ATYPIA
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https://doi.org/10.21272/eumj.2020;8(4):377-382Keywords:
SRA1, rs801460-polymorphism, benign breast disease, thyroid noduleAbstract
Introduction. As it was revealed, the greater part of the human genome is represented by non-coding sequences. They also include long non-coding RNAs (lncRNAs). SRA1 is one of its representatives. This lncRNA affects steroid hormones receptors by activating their transcriptional activity. Thereby, SRA1 can be involved in pathogenesis of steroid-responsive tissues tumors.
Purpose. To study the association between the SRA1 rs801460-polymorphism and the development of thyroid nodules in Ukrainian females with proliferative type of benign breast dysplasia (BBD) without atypia.
Materials and methods. 117 patients with proliferative type of BBD without atypia were enrolled into the study. They were divided into two comparison groups: 12 subjects with thyroid nodules and 105 subjects with no thyroid nodules. All patients were diagnosed on an outpatient basis by a licensed surgeon (license AG No. 600519). The polymerase chain reaction-restriction fragment length polymorphism analysis was used for SRA1 rs801460-polymorphism genotyping. Statistical data processing was performed using Statistical Package for Social Science software (SPSS, version 25.0, Chicago, IL, USA).
Results. The distribution of CC-homozygotes, CT-heterozygotes and TT-homozygotes in patients with thyroid nodules was 1 (8.3 %), 11 (91.7 %) and 0 (0 %), respectively. The distribution of genotypes in the group without thyroid nodules was 38 (36.2 %), 51 (48.6 %) and 16 (15.2 %), respectively. Significant difference was found in genotypes distribution between patients with and without nodules (P = 0.017). Multivariable logistic regression has shown that CT-genotype has lower thyroid nodules risk compared to CC- and TT-genotypes (P = 0.020, OR = 0.083, 95% CI = 0.010-0.681).
Conclusion. A statistically significant association was found between SRA1 rs801460-polymorphism and thyroid nodules occurrence in Ukrainian females with proliferative type of BBD without atypia. Individuals with CT-genotype have less risk of thyroid nodules development compared to homozygotes (CC and TT).
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Copyright (c) 2020 I. M. Lukavenko, A. V. Kolnoguz, M. O. Kyrychenko, O. V. Ataman, V. Yu. Harbuzova
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