BIOMINERALIZATION OF MICROSTRUCTURAL VARIATIONS OF DENTINAL TUBULES AFTER ROOT CANAL OBTURATION USING MTA-BASED CEMENT WITH SUBSEQUENT SEM ANALYSIS
Abstract
Introduction. The quality of endodontic treatment of teeth with intra-root resorption process directly depends on careful instrumental, medicinal treatment and root canal filling. The use of Mineral Trioxide Aggregate (MTA) cement aggregates during root canal obturation is of concern due to its bioactive interactions with dentinal tubule phosphoapatite.
Methods. We prepared 50 curved root canals of human maxillary molars extracted according to indications and prepared them instrumentally for obturation. The intratubular biomineralization at the dentin-filling material interface was carefully analysed by scanning electron microscopy (SEM).
Results.We prepared 50 curved root canals of human maxillary molars extracted according to indications and prepared them instrumentally for obturation. The intratubular biomineralization at the dentin-filling material interface was carefully analysed by scanning electron microscopy (SEM). In the case of root canal obturation with GP and MTA sealer, increased biomineralization of dentinal tubules outside the penetrating sealant mark was confirmed by SEM observation (p<0.05). Mineralised structures of phosphoapatite (calcium/phosphorus ratio 1.45-1.89) were detected on the path of MTA penetration through dentinal tubules at a distance of 350-400 µm from the tubule mouth. The beginnings of crystallisation were observed along the intra- and/or intertubular dentin collagen. The depth of intratubular biomineralization was significantly increased in all pretreated dentinal tubules (p<0.05).
Conclusions. MTA cement and its derivatives can be used as a promising bioactive root canal filling agent to enhance biomineralization of dentinal tubules in a controlled environment.
Downloads
References
Siqueira JF. Strategies to Treat Infected Root Canals. Journal of the California Dental Association [Internet]. 2001 Dec 1;29(12):825–35. Available from: http://dx.doi.org/10.1080/19424396.2001.12223244
Zmener O, Pameijer CH, Serrano SA, Vidueira M, Macchi RL. Significance of Moist Root Canal Dentin with the Use of Methacrylate-based Endodontic Sealers: An In Vitro Coronal Dye Leakage Study. Journal of Endodontics [Internet]. 2008 Jan;34(1):76–9. Available from: http://dx.doi.org/10.1016/j.joen.2007.10.012
Torabinejad M, Parirokh M, Dummer PMH. Mineral trioxide aggregate and other bioactive endodontic cements: an updated overview – part II: other clinical applications and complications. International Endodontic Journal [Internet]. 2017 Oct 11;51(3):284–317. Available from: http://dx.doi.org/10.1111/iej.12843
Tay FR, Pashley DH, Rueggeberg FA, Loushine RJ, Weller RN. Calcium Phosphate Phase Transformation Produced by the Interaction of the Portland Cement Component of White Mineral Trioxide Aggregate with a Phosphate-containing Fluid. Journal of Endodontics [Internet]. 2007 Nov;33(11):1347–51. Available from: http://dx.doi.org/10.1016/j.joen.2007.07.008
Revankar V, Prathap M, Shetty KhK, Shahul A, Sahana K. Effect of biomineralization ability on push-out strength of proroot mineral trioxide aggregate, mineral trioxide aggregate branco, and calcium phosphate cement on dentin: An In vitro evaluation. Journal of Pharmacy And Bioallied Sciences [Internet]. 2017;9(5):121. Available from: http://dx.doi.org/10.4103/jpbs.jpbs_120_17
Gandolfi MG, Siboni F, Primus CM, Prati C. Ion Release, Porosity, Solubility, and Bioactivity of MTA Plus Tricalcium Silicate. Journal of Endodontics [Internet]. 2014 Oct;40(10):1632-7. Available from: http://dx.doi.org/10.1016/j.joen.2014.03.025
Moazami, Fariborz et al. “Evaluation the Solubility and the Porosity of the Nano Fast Cement Comparing to the Mineral Trioxide Aggregate: An in vitro Study.” Journal of dentistry (Shiraz, Iran) vol. 24,1 (2023): 28-33. https://doi.org/10.30476/DENTJODS.2021.92684.1668
Haji TH, Selivany BJ, Suliman AA. Sealing ability in vitro study and biocompatibility in vivo animal study of different bioceramic based sealers. Clinical and Experimental Dental Research [Internet]. 2022 Nov 17;8(6):1582–90. Available from: http://dx.doi.org/10.1002/cre2.652
Abuarqoub D, Aslam N, Jafar H, Abu Harfil Z, Awidi A. Biocompatibility of Biodentine™ ® with Periodontal Ligament Stem Cells: In Vitro Study. Dentistry Journal [Internet]. 2020 Feb 8;8(1):17. Available from: http://dx.doi.org/10.3390/dj8010017
Tavares KIMC, Pinto JC, Santos-Junior AO, Duarte MAH, Guerreiro-Tanomaru JM, Tanomaru-Filho M. Effect of Additional Apical Preparation on Retreatment of Curved Root Canals Filled with Different Sealers. European Journal of Dentistry [Internet]. 2022 Sep 8;17(03):636–41. Available from: http://dx.doi.org/10.1055/s-0042-1750693
Bird DC, Komabayashi T, Guo L, Opperman LA, Spears R. In Vitro Evaluation of Dentinal Tubule Penetration and Biomineralization Ability of a New Root-end Filling Material. Journal of Endodontics [Internet]. 2012 Aug;38(8):1093–6. Available from: http://dx.doi.org/10.1016/j.joen.2012.04.017
Yao S, Jin B, Liu Z, Shao C, Zhao R, Wang X, et al. Biomineralization: From Material Tactics to Biological Strategy. Advanced Materials [Internet]. 2017 Feb 23;29(14). Available from: http://dx.doi.org/10.1002/adma.201605903
Gevkaliuk NO, Sydliaruk NI, Martyts YM, Pynda MY, Krupei VY, Mykhailiuk VM. Differentiation of the structure of dentinal tubules and dentinal canaliculi in human teeth of different ages . Regul. Mech. Biosyst. [Internet]. 2024May27 [cited 2025Jun.26];15(3):394-0. Available from: https://medicine.dp.ua/index.php/med/article/view/1004
Yoo YJ, Lee Y, Yoo J, Perinpanayagam H, Yoo C, Kang H, et al. Intratubular Biomineralization in a Root Canal Filled with Calcium-Enriched Material over 8 Years. Materials [Internet]. 2017 Dec 5;10(12):1388. Available from: http://dx.doi.org/10.3390/ma10121388
This work is licensed under a Creative Commons Attribution 4.0 International License.
ISSN: 
