THE EFFECT OF PLACENTAL CRYOEXTRACT ON THE STATE OF PROTEIN-LIPID METABOLISM IN THE GASTRIC MUCOSA IN EXPERIMENTAL STRESS-INDUCED ULCERS
Introduction. Peptic ulcer is one of the most prevalent diseases of the gastrointestinal tract. Stress factor is considered to have the highest impact on the development of ulcers, as it is present in almost all cases of onset and exacerbation of this disease. Acute stress ulcers occur with severe injuries, acute diseases of various organs, shock, a sharp drop in blood pressure, oxygen deficiency of body tissues, liver, kidney and others. Given the above facts, in the correction of stress-induced lesions of the gastric mucosa (GM) therapeutically, the target should consider not only reducing the aggression factors of gastric juice, but also the normalization of changes in protein and carbohydrate metabolism in GM. In this aspect, our attention was drawn to the domestic biotechnological preparation of placental cryoextract (CEP), which has a range of valuable biological effects.
The aim is to study the effect of cryopreserved placenta extract on the state of protein-lipid metabolism in the gastric mucosa in a model of water-immobilization stress in rats.
Materials and methods of research. The studies were performed on 28 nonlinear laboratory male rats weighing 200–220 g. Stress-induced gastric ulcer was modeled under water-immobilization stress (WIS) in rats according to the method of Takagi et al. To obtain the homogenate, the GM was perfused with cold (+ 4°C) buffer solution and homogenized at 3000 rpm (teflon/glass). The content of oxidatively modificated proteins (OMP) in GM was determined by Dubinina spectrophotometric method. The content of total lipids in the GM was determined spectrophotometrically by color reaction with sulfophosphovaniline reagent. Phospholipids (PL) were fractionated by the method of Svetashev and Vaskovsky.
Research results. Evaluation of changes in protein metabolism in GM showed that the level of total protein in rats, which were preventively administered CEP before WIS, was almost comparable with that of intact rats, i.e., 50.1 ± 1.7 μg/mg tissue and 51.1 ± 1.3 μg/mg of tissue, respectively, which indicated the elimination of disorders of protein homeostasis with the introduction of the studied cryoextract. Studies of changes in total lipids and PL showed that the content of PL of animals treated with CEP was 26.9 ± 0.9%, which was not significantly different from that of intact animals (30.5 ± 0.9%) and, at the same time, it was by 7.3% higher (p < 0.001) as compared with animals who were administered esomeprazole.
Conclusions. Prophylactic five-day administration of CEP to WIS leads to normalization of all evaluated indices, in particular, to increase of the total protein level by 29.0% (p <0.01), decrease of oxidatively modified proteins level by 20.6% (p <0.01), and the 2.3-fold increase (p <0.001) of the level of phospholipids in the total lipids pool.
2. Bereda G. Peptic Ulcer Disease: Definition, Pathophysiology, and Treatment. Journal of Biomedical and Biological Sciences. 2022;1(2):1–10.
3. Pandey A, Saraswat N, Wal P, Pal RS, Wal A, Maurya DM. A detailed review on: recent advances, pathophysiological studies and mechanism of peptic ulcer. Research Journal of Pharmacology and Pharmacodynamics. 2019;11(4):165–70. DOI: https://doi.org/10.5958/2321-5836.2019.00029.6.
4. Shell EJ. Pathophysiology of peptic ulcer disease. Physician Assistant Clinics. 2021;6(4):603–11. DOI: https://doi.org/10.1016/j.cpha.2021.05.005.
5. Pan SY, Chan MKS, Wong MBF, Klokol D, Chernykh V. Placental therapy: An insight to their biological and therapeutic properties. Journal of Medicine and Therapeutics. 2017;1(3):1–6. DOI: http://doi.org/10.15761/JMT.1000118.
6. Holtsev AN, Yurchenko TN, ed., Blazhko EV, Bobyreva LE, Heraskyna LR, Hryshchenko VY, Hubyna-Vakulyk HY, Dvornyk YL, Evtereva YA, Zhdan VN, Zvarych PR, Kapustianskaia AA, Kuzmyna YIu, Lypyna OV, Lomakova YV, Lutsenko NS, Muryzyna YIu, Plotnykova VN, Prokopiuk VIu, Prokopenko OS, Reznykova VA, Strona VY, Strona DV, Tryfanov VIu, Feskova AM, Feskova YA, Shepytko VY, Shepytko KV. Placenta: cryopreservation, clinical use. Kharkiv: Brovyn AV; 2013. 268 p.
7. Pogozhykh O, Prokopyuk V, Figueiredo C, Pogozhykh D. Placenta and placental derivatives in regenerative therapies: experimental studies, history, and prospects. Stem Cells International. 2018;2018:1–14. DOI: https://doi.org/10.1155/2018/4837930.
8. Hladkykh FV. Modulation of meloxicam-induced changes in gastrointestinal and motor activity of the stomach by applying placenta cryoextract. Proceedings of the Shevchenko Scientific Society. Medical Sciences. 2021; 61(1):84–94. DOI: https://doi.org/10.25040/ntsh2021.01.08.
9. Hladkykh FV. Gastrocytoprotective properties of cryopreserved placenta extract in combined action of low temperatures and inhibition of cyclooxygenase. Acta Facultatis Medicae Naissensis. 2022;39(1):48–56. DOI: https://doi.org/10.5937/afmnai39-33036.
10. Rybolovlev UR, Rybolovlev RS. Dosage of substances for mammals by constants of biological activity. Reports of the USSR Academy of Sciences. 1979;247(6):1513–6.
11. Wei Xie, Xielin Huang, Renpin Chen, Ruru Chen, Tang Li, Wei Wu, Zhiming Huang. Esomeprazole alleviates the damage to stress ulcer in rats through not only its antisecretory effect but its antioxidant effect by inactivating the p38 MAPK and NF-κB signaling pathways. Drug Design, Development and Therapy. 2019;22(13):2969–84. DOI: http://doi.org/10.2147/DDDT.S193641.
12. Stefanov OV. Preclinical studies of drugs: guidelines. Kyiv: Avicenna; 2001. 527 p.
13. Takagi KY, Kayuya Y, Watanabe K. Studies on drugs for peptic ulcer. A reliable method for producing stress ulcers in rats. Chemical and Pharmaceutical Bulletin. 1964;12:465–72. DOI: http://doi.org/10.1248/cpb.12.465.
14. Kamyshnikov VS. Handbook of clinical and biochemical research and laboratory diagnostics. MEDpress-inform; 2009. 896 p.
15. Dubinina EE, Pustigina AV. Oxidative modification of proteins, its role in pathological conditions. Ukrainian Biochemical Journal. 2008;80(6):5–15.
16. Bligh EG, Dyer WI. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology. 1959;37(8):911–7.
17. Svetashev VI, Vaskovsky VE. A simplified technique for thin layer microchromatography of lipids. Journal of Chromatography. 1972;67:376–8.
18. Vaskovsky VE, Kostetsky EY, Vasendin IM. A universal reagent for phospholipid analysis. Journal of Chromatography. 1975:114:129–41.
19. Makovetskaya LI, Sarnatskaya VV. Biomarkers of oxidative carbonyl stress in rats with gerenic carcinoma in the progression of tumor development depending on the sensitivity to cisplatin. Oncology. 2021;23(3):83–92. DOI: http://doi.org/10.32471/oncology.2663-7928.t-23-3-2021-g.9805
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